Liquid and solid-state Cl- -sensitive microelectrodes. Characteristics and application to intracellular Cl- activity in Balanus photoreceptor.

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Liquid and solid-state Cl- -sensitive microelectrodes. Characteristics and application to intracellular Cl- activity in Balanus photoreceptor

When intracellular chloride activity (aiCl) was monitored with chloride-sensitive liquid ion exchanges (CLIX) microelectrodes in Balanus photoreceptors, replacement of extracellular chloride (Cl0) by methanesulfonate or glutamate was followed by a rapid but incomplete loss of aiCl. When propionate was used as the extracellular anion substitute, CLIX electrodes detected an apparent gain in aiCl,...

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Liquid and Solid-State Cl--Sensitive Microelectrodes Characteristics and Application to Intracellular C1- Activity in Balanus Photoreceptor

I N T R O D U C T I O N Using chloride liquid ion exchanger (CLIX) microelectrodes similar to those described by O r m e (1969) and Walker (1971), Brown showed (1976) that chloride was passively distributed in the dark-adapted barnacle photoreceptor , i.e. he repor ted a range o f values for the internal chloride activity (a~l) and he noted that the calculated Ecl was generally within I-2 mV of...

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Liquid and Solid-State Cl--Sensitive Microelectrodes Characteristics and Application to Intracellular C1- Activity in Balanus Photoreceptor

I N T R O D U C T I O N Using chloride liquid ion exchanger (CLIX) microelectrodes similar to those described by O r m e (1969) and Walker (1971), Brown showed (1976) that chloride was passively distributed in the dark-adapted barnacle photoreceptor , i.e. he repor ted a range o f values for the internal chloride activity (a~l) and he noted that the calculated Ecl was generally within I-2 mV of...

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A furosemide-sensitive K+-Cl- cotransporter counteracts intracellular Cl- accumulation and depletion in cultured rat midbrain neurons.

Efficacy of postsynaptic inhibition through GABAA receptors in the mammalian brain depends on the maintenance of a Cl- gradient for hyperpolarizing Cl- currents. We have taken advantage of the reduced complexity under which Cl- regulation can be investigated in cultured neurons as opposed to neurons in other in vitro preparations of the mammalian brain. Tightseal whole-cell recording of spontan...

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Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl- channels in human osteoblasts.

The whole cell recording mode of the patch-clamp technique was used to study the effect of hypotonic NaCl or isotonic high-KCl solution on membrane currents in a human osteoblast-like cell line, C1. Both hypotonic NaCl or isotonic high-KCl solution activated Cl- channels expressed in these cells as described previously. The reversal potential of the induced Cl- current is more negative when act...

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 1977

ISSN: 0022-1295,1540-7748

DOI: 10.1085/jgp.70.4.507